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bioRxiv · 10.1101/2021.06.30.450524

Horizontal Transfer of Histone H3 by Mammalian Cells

Abstract

The authors have withdrawn their manuscript because many of the experiments described in this paper have not been reproducible, or at least are not robust, in the hands of other members of the Kaelin Laboratory who were not initially involved in this work. While we do see apparent secretion of histone H3 under some conditions, it is usually accompanied by secretion of histone H4. In this regard, the Halo tagged-histone H3 and Halo-tagged H4 constructs used for the single molecule imaging studies we reported, which seemingly confirmed specific secretion and transfer of histone H3, were purported to be sequence validated. Upon resequencing these constructs we discovered a non-synonymous mutation in the Halo tag of the H4 construct. We then redid the imaging experiments with the corrected Halo-H4 together with Halo-H3 and, in contrast to our earlier study, unfused Halo. These experiments were difficult to interpret because of the background signal seen with the unfused Halo but did not support specific secretion and transfer of histones (let alone specific secretion and transfer of histone H3). We have, in some experiments, observed transfer of H3-Cre into reporter cells ex vivo and in vivo, but 1) the transfer is not specific for H3-Cre versus H4-Cre, 2) the transfer efficiency is highly variable, and 3) the transfer efficiency is typically much less than reported in our original paper. We do not yet know whether this lack of reproducibility and robustness reflects technical and biological variables that we do not yet understand and hence were not captured in our experimental protocols. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.

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BibTeXRIS

Sulkowski, P. L., Nicholson, H. E., Li, M. Z., Perrimon, N., Liu, Z., Kaelin, W. G.. 2021-06-30. Horizontal Transfer of Histone H3 by Mammalian Cells. https://doi.org/10.1101/2021.06.30.450524

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