High-throughput single-cell TCR - pMHC dissociation rate measurements performed by an autonomous microfluidic cellular processing unit

Jammes, F.; Schmidt, J.; Coukos, G.; Maerkl, S. J.

doi:10.1101/2021.06.30.450499

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bioRxiv · 10.1101/2021.06.30.450499

High-throughput single-cell TCR - pMHC dissociation rate measurements performed by an autonomous microfluidic cellular processing unit

Jammes, F.·Schmidt, J.·Coukos, G.·Maerkl, S. J.

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Abstract

We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells, perform, measure, and on-the-fly analyze cell-surface dissociation rates, followed by recovery of selected cells. We performed proof-of-concept, high-throughput single-cell experiments characterizing pMHC-TCR interactions on live CD8+ T cells. The mCPU platform analyzed TCR-pMHC dissociation rates with a throughput of 50 cells per hour and hundreds of cells per run, and we demonstrate that cells can be selected, enriched, and easily recovered from the device.

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Jammes, F., Schmidt, J., Coukos, G., Maerkl, S. J.. 2021-07-01. High-throughput single-cell TCR - pMHC dissociation rate measurements performed by an autonomous microfluidic cellular processing unit. https://doi.org/10.1101/2021.06.30.450499

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