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bioRxiv · 10.1101/2021.06.24.449705

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

Kumagai, Y.·Liu, Y.·Hamada, H.·Luo, W.·Zhu, J.·Kuroki, M.·Nagira, Y.·Taoka, N.·Kato, E.·Imai, R.
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Abstract

In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant to culture-based transformation methods. Here, we report the direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem using in planta particle bombardment and introduction of a semidwarf1 (sd1)-orthologous mutation into wheat. The triple knockout tasd1 mutant of an elite wheat variety reduced culm length by 10% without a reduction in yield.

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Kumagai, Y., Liu, Y., Hamada, H., Luo, W., Zhu, J., Kuroki, M., Nagira, Y., Taoka, N., Kato, E., Imai, R.. 2021-06-24. In planta genome editing with CRISPR/Cas9 ribonucleoproteins. https://doi.org/10.1101/2021.06.24.449705

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