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bioRxiv · 10.1101/2021.06.23.449625

High quality, phased genomes of Phytophthora ramorum clonal lineages NA1 and EU1

Carleson, N. C.·Press, C. M.·Grunwald, N. J.
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Abstract

Phytophthora ramorum is the causal agent of sudden oak death in West Coast forests and currently two clonal lineages, NA1 and EU1, cause epidemics in Oregon forests. Here, we report on two high-quality genomes of individuals belonging to the NA1 and EU1 clonal lineages respectively, using PacBio long-read sequencing. The NA1 strain Pr102, originally isolated from coast live oak in California, is the current reference genome and was previously sequenced independently using either Sanger (P. ramorum v1) or PacBio (P. ramorum v2) technology. The EU1 strain PR-15-019 was obtained from tanoak in Oregon. These new genomes have a total size of 57.5 Mb, with a contig N50 length of ~3.5-3.6 Mb and encode ~15,300 predicted protein-coding genes. Genomes were assembled into 27 and 28 scaffolds with 95% BUSCO scores and are considerably improved relative to the current JGI reference genome with 2,575 or the PacBio genomes with 1,512 scaffolds. These high-quality genomes provide a valuable resource for studying the genetics, evolution, and adaptation of these two clonal lineages.

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Carleson, N. C., Press, C. M., Grunwald, N. J.. 2021-06-23. High quality, phased genomes of Phytophthora ramorum clonal lineages NA1 and EU1. https://doi.org/10.1101/2021.06.23.449625

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