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bioRxiv · 10.1101/2021.06.22.449452

Dystonia-specific mutations in THAP1 alter transcription of genes associated with neurodevelopment and myelin

Abstract

Dystonia is a neurologic disorder associated with an increasingly large number of variants in many genes, resulting in characteristic disturbances in volitional movement. Dissecting the relationships between these mutations and their functional outcomes is a critical step in understanding the key pathways that drive dystonia pathogenesis. Here we established a pipeline for characterizing an allelic series of dystonia-specific mutations in isogenic induced pluripotent stem cells (iPSCs). We used this strategy to investigate the molecular consequences of variation in THAP1, which encodes a transcription factor that has been linked to neural differentiation. Multiple pathogenic mutations that have been associated with dystonia cluster within distinct THAP1 functional domains and are predicted to alter its DNA binding properties and/or protein interactions differently, yet the relative impact of these varied changes on molecular signatures and neural deficits is unclear. To determine the effects of these mutations on THAP1 transcriptional activity, we engineered an allelic series of eight mutations in a common iPSC background and differentiated these lines into a panel of near-isogenic neural stem cells (n = 94 lines). Transcriptome profiling of these neural derivatives followed by joint analysis of the most robust individual signatures across mutations identified a convergent pattern of dysregulated genes functionally related to neurodevelopment, lysosomal lipid metabolism, and myelin. Based on these observations, we examined mice bearing Thap1-disruptive alleles and detected significant changes in myelin gene expression and reduction of myelin structural integrity relative to tissue from control mice. These results suggest that deficits in neurodevelopment and myelination are common consequences of dystonia-associated THAP1 mutations and highlight the potential role of neuron-glial interactions in the pathogenesis of dystonia.

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BibTeXRIS

Domingo, A., Yadav, R., Shah, S., Hendriks, W. T., Erdin, S., Gao, D., O'Keefe, K., Currall, B., Gusella, J. F., Sharma, N., Ozelius, L. J., Ehrlich, M. E., Talkowski, M. E., Bragg, D. C.. 2021-06-22. Dystonia-specific mutations in THAP1 alter transcription of genes associated with neurodevelopment and myelin. https://doi.org/10.1101/2021.06.22.449452

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