Rapid Genomic Characterization and Global Surveillance of Klebsiella Using Pathogenwatch

Argimon, S.; David, S.; Underwood, A.; Abrudan, M.; Wheeler, N. E.; Kekre, M.; Abudahab, K.; Yeats, C. A.; Goater, R.; Taylor, B.; Harste, H.; Muddyman, D.; Feil, E. J.; Brisse, S.; Holt, K.; Donado-Godoy, P.; Ravikumar, K. L.; Okeke, I. N.; Carlos, C. C.; Aanensen, D. M.

doi:10.1101/2021.06.22.448967

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bioRxiv · 10.1101/2021.06.22.448967

Rapid Genomic Characterization and Global Surveillance of Klebsiella Using Pathogenwatch

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Abstract

BackgroundKlebsiella species, including the notable pathogen K. pneumoniae, are increasingly associated with antimicrobial resistance (AMR). Genome-based surveillance can inform interventions aimed at controlling AMR. However, its widespread implementation requires tools to streamline bioinformatic analyses and public health reporting. MethodsWe developed the web application Pathogenwatch, which implements analytics tailored to Klebsiella species for integration and visualization of genomic and epidemiological data. We populated Pathogenwatch with 16,537 public Klebsiella genomes to enable contextualization of user genomes. We demonstrated its features with 1,636 genomes from four low- and middle-income countries (LMICs) participating in the NIHR Global Health Research Unit (GHRU) on AMR. ResultsUsing Pathogenwatch, we found that GHRU genomes were dominated by a small number of epidemic drug-resistant clones of K. pneumoniae. However, differences in their distribution were observed (e.g. ST258/512 dominated in Colombia, ST231 in India, ST307 in Nigeria, ST147 in the Philippines). Phylogenetic analyses including public genomes for contextualization enabled retrospective monitoring of their spread. In particular, we identified hospital outbreaks, detected introductions from abroad, and uncovered clonal expansions associated with resistance and virulence genes. Assessment of loci encoding O-antigens and capsule in K. pneumoniae, which represent possible vaccine candidates, showed that three O-types (O1-O3) represented 88.9% of all genomes, whereas capsule types were much more diverse. ConclusionsPathogenwatch provides a free, accessible platform for real-time analysis of Klebsiella genomes to aid surveillance at local, national and global levels. We have improved representation of genomes from GHRU participant countries, further facilitating ongoing surveillance. summaryPathogenwatch is a free web-application for analysis of Klebsiella genomes to aid surveillance at local, national and global levels. We improved the representation of genomes from middle-income countries through the Global Health Research Unit on AMR, further facilitating ongoing surveillance.

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Argimon, S., David, S., Underwood, A., Abrudan, M., Wheeler, N. E., Kekre, M., Abudahab, K., Yeats, C. A., Goater, R., Taylor, B., Harste, H., Muddyman, D., Feil, E. J., Brisse, S., Holt, K., Donado-Godoy, P., Ravikumar, K. L., Okeke, I. N., Carlos, C. C., Aanensen, D. M.. 2021-06-22. Rapid Genomic Characterization and Global Surveillance of Klebsiella Using Pathogenwatch. https://doi.org/10.1101/2021.06.22.448967

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