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bioRxiv · 10.1101/2021.06.16.448624

In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSCmed cells

PUIG LOMBARDI, E.·BAURES, M.·DARIANE, C.·GUIDOTTI, J.-E.·Goffin, V.
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Abstract

Several groups recently published single-cell (sc) expression atlases of the adult mouse prostate cells based on RNA sequencing (scRNA-seq) data. All studies identified one computerized cluster of non-secretory luminal progenitor cells enriched in luminal and stemness-related gene transcripts. The actual correspondence between these luminal progenitor cell clusters has not been investigated. In addition, the presence of Krt4 (encoding cytokeratin 4) in these in silico-identified luminal progenitors suggested the overlap with FACS-enriched LSCmed luminal progenitor cells earlier identified as a stem-like, castration-tolerant and tumor-initiating cell population. Here, we used a unified bioinformatics pipeline to re-analyze published prostate scRNA-seq datasets and perform various pan-transcriptomic comparisons including the LSCmed cell signature. Our study demonstrates that i) the mouse prostate luminal progenitor cell clusters identified in the different scRNA-seq studies largely overlap and can be defined by a common 15-gene signature including Krt4, ii) mouse LSCmed cells match both mouse and human luminal progenitors identified by scRNA-seq analysis. Bridging these in silico-identified and ex vivo-characterized prostate luminal progenitor subsets should benefit our understanding of their actual involvement in prostate diseases.

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PUIG LOMBARDI, E., BAURES, M., DARIANE, C., GUIDOTTI, J.-E., Goffin, V.. 2021-06-17. In silico Computed Clusters of Prostate Luminal Progenitors Match FACS-Enriched LSCmed cells. https://doi.org/10.1101/2021.06.16.448624

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