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bioRxiv · 10.1101/2021.06.11.448119

Structural Basis for Cytoplasmic Dynein-1 Regulation by Lis1

Gillies, J. P.·Reimer, J. M.·Karasmanis, E. P.·Lahiri, I.·Htet, Z. M.·Leschziner, A. E.·Reck-Peterson, S. L.
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Abstract

The lissencephaly 1 gene, LIS1, is mutated in patients with the neurodevelopmental disease lissencephaly. The Lis1 protein is conserved from fungi to mammals and is a key regulator of cytoplasmic dynein-1, the major minus-end-directed microtubule motor in many eukaryotes. Lis1 is the only dynein regulator that binds directly to dyneins motor domain, and by doing so alters dyneins mechanochemistry. Lis1 is required for the formation of fully active dynein complexes, which also contain essential cofactors: dynactin and an activating adaptor. Here, we report the first high-resolution structure of the yeast dynein-Lis1 complex. Our 3.1[A] structure reveals, in molecular detail, the major contacts between dynein and Lis1 and between Lis1s {beta}-propellers. Structure-guided mutations in Lis1 and dynein show that these contacts are required for Lis1s ability to form fully active human dynein complexes and to regulate yeast dyneins mechanochemistry and in vivo function. We present a model for the conserved role of Lis1 in regulating dynein from yeast to humans.

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Gillies, J. P., Reimer, J. M., Karasmanis, E. P., Lahiri, I., Htet, Z. M., Leschziner, A. E., Reck-Peterson, S. L.. 2021-06-11. Structural Basis for Cytoplasmic Dynein-1 Regulation by Lis1. https://doi.org/10.1101/2021.06.11.448119

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