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bioRxiv · 10.1101/2021.06.08.447622

An Efficient and Cost-effective Purification Methodology for Cas9 Nuclease

Teng, A. C. T.·Tavassoli, M.·Shrestha, S.·Lindsay, K.·Ivakine, E.·Cohn, R.·Santerre, J. P.·Gramolini, A. O.
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Abstract

With an ever-increasing demand for laboratory-grade Cas9 proteins by many groups advancing the use of CRISPR technology, a more efficient and scalable process for generating the proteins, coupled with rapid purification methods is in urgent demand. Here, we introduce a modified methodology for rapid purification of active SaCas9 protein within 24 hours. The product has over 90% protein purity. The simplicity and cost-effectiveness of such methodology will enable general labs to produce a sizable amount of Cas9 proteins, further accelerating the advancement of CRISPR/Cas9-based research.

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Teng, A. C. T., Tavassoli, M., Shrestha, S., Lindsay, K., Ivakine, E., Cohn, R., Santerre, J. P., Gramolini, A. O.. 2021-06-08. An Efficient and Cost-effective Purification Methodology for Cas9 Nuclease. https://doi.org/10.1101/2021.06.08.447622

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