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bioRxiv · 10.1101/2021.06.02.446858

The RNA helicase Ded1 regulates translation and granule formation during multiple phases of cellular stress responses

Abstract

Ded1 is a conserved RNA helicase that promotes translation initiation in steady-state conditions. Ded1 has also been shown to regulate translation during cellular stress and affect the dynamics of stress granules (SGs), accumulations of RNA and protein linked to translation repression. To better understand its role in stress responses, we examined Ded1 function in two different models: DED1 overexpression and oxidative stress. DED1 overexpression inhibits growth and promotes the formation of SGs. A ded1mutant lacking the low-complexity C-terminal region (ded1-{Delta}CT), which mediates Ded1 oligomerization and interaction with the translation factor eIF4G, suppressed these phenotypes, consistent with other stresses. During oxidative stress, a ded1-{Delta}CT mutant was defective in growth and in SG formation compared to wild-type cells, although SGs were increased rather than decreased in these conditions. Unlike stress induced by direct TOR inhibition, the phenotypes in both models were only partially dependent on eIF4G interaction, suggesting an additional contribution from Ded1 oligomerization. Furthermore, examination of the growth defects and translational changes during oxidative stress suggested that Ded1 plays a role during recovery from stress. Integrating these disparate results, we propose that Ded1 controls multiple aspects of translation and RNP dynamics in both initial stress responses and during recovery.

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BibTeXRIS

Aryanpur, P. P., Mittelmeier, T. M., Bolger, T. A.. 2021-06-03. The RNA helicase Ded1 regulates translation and granule formation during multiple phases of cellular stress responses. https://doi.org/10.1101/2021.06.02.446858

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