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bioRxiv · 10.1101/2021.06.02.446743

Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni

Lasica, C.·de Carvalho Augusto, R.·Mone, H.·Mouahid, G.·Chaparro, C.·Veillard, A.-C.·Zelisko-Schmidt, A.·Grunau, C.
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Abstract

Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni, whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit [≥]20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.

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Lasica, C., de Carvalho Augusto, R., Mone, H., Mouahid, G., Chaparro, C., Veillard, A.-C., Zelisko-Schmidt, A., Grunau, C.. 2021-06-02. Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni. https://doi.org/10.1101/2021.06.02.446743

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