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bioRxiv · 10.1101/2021.05.25.445583

Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand

Siriwan, W.·Saokham, K.·Hemniam, N.·Roekwan, S.·Hunsawattanakul, S.·Thawinampan, J.
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Abstract

Cassava plantations in an area of 458 ha spanning five provinces along the Thailand- Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease severity was generally scored as 2-3. The highest average disease severity was in Sakaeo province (3.7), followed by Buriram (3.6), Prachinburi (2.88), Surin (2.5), and Sisaket (2.4) provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Interestingly, cassava cultivars CMR-89 and Rayong 11 were susceptible to CMD. In approximately 95% of cases, the infection was transmitted by whitefly (Bemisia tabaci), which had a high population density in Prachinburi but was sparse in Surin, with the largest populations observed in May and June. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whitefly (Bemisia tabaci) in Thailand revealed a similarity to the Asia II 1 whitefly gene. Furthermore, the AV1 gene--which encodes the capsid protein--showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.

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Siriwan, W., Saokham, K., Hemniam, N., Roekwan, S., Hunsawattanakul, S., Thawinampan, J.. 2021-05-25. Survey and Molecular Detection of Sri Lankan Cassava Mosaic Virus in Thailand. https://doi.org/10.1101/2021.05.25.445583

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