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bioRxiv · 10.1101/2021.05.24.445390

Extremely potent human monoclonal antibodies for the prophylaxis and therapy of Tetanus

Abstract

Human monoclonal antibodies were used here to study the mechanism of neuron intoxication by tetanus neurotoxin protein toxins and as a safe preventive and therapeutic substitute of hyperimmune sera. By screening memory B cells of immune donors, we selected two monoclonal antibodies specific for tetanus neurotoxin with exceptionally high neutralizing activities, which have been extensively characterized both structurally and functionally. We found that these antibodies interfere with the binding and translocation of the neurotoxin into neurons by interacting with two epitopes, whose definition pinpoints crucial events in the cellular pathogenesis of tetanus. Some mechanistic aspects of tetanus neurotoxin intoxication were revealed, explaining at the same time, the unprecedented neutralization ability of these antibodies. Importantly, these antibodies are exceptionally efficient in preventing experimental tetanus when injected in mice long before the neurotoxin. Moreover, their Fab derivatives neutralize tetanus neurotoxin in post-exposure experiments, suggesting their potential therapeutic use upon intrathecal injection. As such, these human monoclonal antibodies, as well as their Fab derivatives, meet all requirements for being considered for prophylaxis and therapy of human tetanus and are ready for clinical trials.

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BibTeXRIS

Pirazzini, M., Grinzato, A., Corti, D., Barbieri, S., Leka, O., Vallese, F., Tonellato, M., Silacci Fregni, C., Piccoli, L., Kandiah, E., Schiavo, G., Zanotti, G., Lanzavecchia, A., Montecucco, C.. 2021-05-25. Extremely potent human monoclonal antibodies for the prophylaxis and therapy of Tetanus. https://doi.org/10.1101/2021.05.24.445390

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