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bioRxiv · 10.1101/2021.05.20.444935

Fluorescence signatures of SARS CoV-2 spike S1 proteins and an human ACE-2: excitation-emission maps and fluorescence lifetimes

Grzesiak, J.·Fellner, L.·Grünewald, K.·Kölbl, C.·Walter, A.·Horlacher, R.·Duschek, F.
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Abstract

SignificanceFast and reliable detection of infectious SARS-CoV-2 virus loads is an important issue. Fluorescence spectroscopy is a sensitive tool to do so in clean environments. This presumes a comprehensive knowledge of fluorescence data. AimThis work aims at providing fully featured information on wavelength and time-dependent data of the fluorescence of the SARS-CoV-2 spike protein S1 subunit, its receptor binding domain (RBD) and the human angiotensinconverting enzyme 2 (hACE2), especially with respect to possible optical detection schemes. ApproachSpectrally resolved excitation-emission maps of the involved proteins and measurements of fluorescence lifetimes were recorded for excitations from 220 to 295 nm. The fluorescence decay times were extracted by using a bi-exponential kinetic approach. The binding process in the SARS-CoV-2 RBD was likewise examined for spectroscopic changes. ResultsDistinct spectral features for each protein are pointed out in relevant spectra extracted from the excitation emission maps. We also identify minor spectroscopic changes under the binding process. The decay times in the bi-exponential model are found to be (2.0{+/-} 0.1) ns and (8.0 {+/-}1.0) ns. ConclusionsSpecific material data serve as important background information for the design of optical detection and testing methods for SARS-CoV-2 loaded media.

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Grzesiak, J., Fellner, L., Grünewald, K., Kölbl, C., Walter, A., Horlacher, R., Duschek, F.. 2021-05-20. Fluorescence signatures of SARS CoV-2 spike S1 proteins and an human ACE-2: excitation-emission maps and fluorescence lifetimes. https://doi.org/10.1101/2021.05.20.444935

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