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bioRxiv · 10.1101/2021.05.13.444041

An ultrasensitive method for analysis of viral spike N-glycoforms

Baboo, S.·Diedrich, J. K.·Martinez-Bartolome, S.·Wang, X.·Schiffner, T.·Groschel, B.·Schief, W. R.·Paulson, J. C.·Yates, J. R.
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Abstract

Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for analysis of site-specific glycosylation of any glycoprotein.

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Baboo, S., Diedrich, J. K., Martinez-Bartolome, S., Wang, X., Schiffner, T., Groschel, B., Schief, W. R., Paulson, J. C., Yates, J. R.. 2021-05-15. An ultrasensitive method for analysis of viral spike N-glycoforms. https://doi.org/10.1101/2021.05.13.444041

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