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bioRxiv · 10.1101/2021.05.11.443581

The macrophage reprogramming ability of antifolates reveals soluble CD14 as a potential biomarker for methotrexate response in rheumatoid arthritis

Abstract

The physio-pathological relevance of the one-carbon metabolism (OCM) is illustrated by the chemotherapeutic and anti-inflammatory effects of the antifolates Pemetrexed (PMX) and Methotrexate (MTX) in cancer and rheumatoid arthritis (RA). We report that OCM determines the functional and gene expression profile of human macrophages. PMX induces the acquisition of a p53-dependent proinflammatory gene signature in human monocyte-derived macrophages (GM-MO). Indeed, OCM blockade reprograms GM-MO towards a state of LPS-tolerance at the signaling and functional levels, an effect abolished by folinic acid. Importantly, OCM blockade led to reduced expression of membrane-bound and soluble CD14 (sCD14), whose exogenous addition restores LPS sensitivity. The therapeutic relevance of these results was confirmed in early RA patients, as MTX-responder RA patients exhibit lower sCD14 serum levels, with baseline sCD14 levels predicting MTX response. Our results indicate that OCM is a metabolic circuit that critically mediates the acquisition of innate immune tolerance, and positions sCD14 as a valuable tool to predict MTX-response in RA patients.

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BibTeXRIS

Fuentelsaz-Romero, S., Barrio Alonso, C., Garcia Campos, R., Torres Torresano, M., Muller, I., Triguero-Martinez, A., Nuno, L., Villalba, A., Garcia-Vicuna, R., Jansen, G., Miranda-Carus, M. E., Gonzalez-Alvaro, I., Puig-Kröger, A.. 2021-05-11. The macrophage reprogramming ability of antifolates reveals soluble CD14 as a potential biomarker for methotrexate response in rheumatoid arthritis. https://doi.org/10.1101/2021.05.11.443581

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