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bioRxiv · 10.1101/2021.05.07.443176

A Mean Shift Algorithm for Drift Correction in Localization Microscopy

Fazekas, F. J.·Shaw, T. R.·Kim, S.·Bogucki, R. A.·Veatch, S. L.
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Abstract

Single molecule localization microscopy (SMLM) techniques transcend the diffraction limit of visible light by localizing isolated emitters sampled stochastically. This time-lapse imaging necessitates long acquisition times, over which sample drift can become large relative to the localization precision. Here we present a novel, efficient, and robust method for estimating drift using a simple peak-finding algorithm based on mean shifts that is effective for SMLM in 2 or 3 dimensions.

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Fazekas, F. J., Shaw, T. R., Kim, S., Bogucki, R. A., Veatch, S. L.. 2021-05-07. A Mean Shift Algorithm for Drift Correction in Localization Microscopy. https://doi.org/10.1101/2021.05.07.443176

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