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bioRxiv · 10.1101/2021.05.06.441257

Vps501, a novel vacuolar SNX-BAR protein cooperates with the SEA complex to induce autophagy

Goyal, S.·Segarra, V. A.·Stecher, A. M.·Nitika,·Truman, A. W.·Reitzel, A. M.·Chi, R. J.
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Abstract

The sorting nexins (SNX), constitute a diverse family of molecules that play varied roles in membrane trafficking, cell signaling, membrane remodeling, organelle motility and autophagy. In particular, the SNX-BAR proteins, a SNX subfamily characterized by a C-terminal dimeric Bin/Amphiphysin/Rvs (BAR) lipid curvature domain and a conserved Phox-homology domain, are of great interest. In budding yeast, many SNX-BARs proteins have well-characterized endo-vacuolar trafficking roles. Phylogenetic analyses allowed us to identify an additional SNX-BAR protein, Vps501, with a novel endo-vacuolar role. We report that Vps501 uniquely localizes to the vacuolar membrane and works with the SEA complex to regulate autophagy. Furthermore, we found cells displayed a severe deficiency in starvation-induced/nonselective autophagy only when SEA complex subunits are ablated in combination with Vps501, indicating a cooperative role with the SEA complex during autophagy. Additionally, we found the SEA complex becomes destabilized in vps501{Delta}sea1{Delta} cells, which resulted in aberrant TORC1 hyperactivity and misregulation of autophagy induction.

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Goyal, S., Segarra, V. A., Stecher, A. M., Nitika,, Truman, A. W., Reitzel, A. M., Chi, R. J.. 2021-05-06. Vps501, a novel vacuolar SNX-BAR protein cooperates with the SEA complex to induce autophagy. https://doi.org/10.1101/2021.05.06.441257

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