The Taxus genome provides insights into paclitaxel biosynthesis

Xiong, X.; Gou, J.; Liao, Q.; Li, Y.; Zhou, Q.; Bi, G.; Li, C.; Du, R.; Wang, X.; Sun, T.; Guo, L.; Liang, H.; Lu, P.; Wu, Y.; Zhang, Z.; Ro, D.-K.; Shang, Y.; Huang, S.; Yan, J.

doi:10.1101/2021.04.29.441981

bioRxiv · 10.1101/2021.04.29.441981

The Taxus genome provides insights into paclitaxel biosynthesis

Xiong, X.·Gou, J.·Liao, Q.·Li, Y.·Zhou, Q.·Bi, G.·Li, C.·Du, R.·Wang, X.·Sun, T.·Guo, L.·Liang, H.·Lu, P.·Wu, Y.·Zhang, Z.·Ro, D.-K.·Shang, Y.·Huang, S.·Yan, J.

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Abstract

The ancient gymnosperm genus Taxus is the exclusive source of the anticancer drug paclitaxel, yet no reference genome sequences are available for comprehensively elucidating the paclitaxel biosynthesis pathway. We have completed a chromosome-level genome of Taxus chinensis var. mairei with a total length of 10.23 Gb. Taxus shared an ancestral whole-genome duplication with the coniferophyte lineage and underwent distinct transposon evolution. We discovered a unique physical and functional grouping of CYP725As (cytochrome P450) in the Taxus genome for paclitaxel biosynthesis. We also identified a gene cluster in the taxadiene biosynthesis, which was mainly formed by gene duplications. This study will facilitate the elucidation of paclitaxel biosynthesis and unleash the biotechnological potential of Taxus. One Sentence SummaryA chromosome-level genome assembly of Taxus chinensis var. mairei uncovers its unique genome evolution process and genetic architectures for the paclitaxel biosynthesis pathway.

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Xiong, X., Gou, J., Liao, Q., Li, Y., Zhou, Q., Bi, G., Li, C., Du, R., Wang, X., Sun, T., Guo, L., Liang, H., Lu, P., Wu, Y., Zhang, Z., Ro, D.-K., Shang, Y., Huang, S., Yan, J.. 2021-04-29. The Taxus genome provides insights into paclitaxel biosynthesis. https://doi.org/10.1101/2021.04.29.441981

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