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bioRxiv · 10.1101/2021.04.26.441486

PEAR: a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

Simon, D. A.·Talas, A.·Kulcsar, P. I.·Welker, E.
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Abstract

Prime editing is a recently developed gene engineering tool that allows the introduction of short insertions, deletions or substitutions into the genome. However, the efficiency of prime editing, generally reaching around 10-30% editing, has not resembled its versatility. Here, Prime Editor Activity Reporter (PEAR), a sensitive fluorescent tool is introduced for the identification of single cells with prime editing activity. Possessing no background fluorescence, PEAR specifically reports on prime editing events in individual cells. By design, it ensures unrestricted flexibility for sequence variations in the full length of the target sequence. The application of PEAR as an enrichment marker of prime editing can increase the edited population by up to 70% and alleviate the burden of the otherwise time and labour consuming process of cloning of the correctly edited cells, therefore considerably improving the applicability of prime editing in fundamental research and biotechnological uses.

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Simon, D. A., Talas, A., Kulcsar, P. I., Welker, E.. 2021-04-27. PEAR: a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells. https://doi.org/10.1101/2021.04.26.441486

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