SIX1 is a master regulator of the Rhabdomyosarcoma undifferentiated state

Hsu, J. Y.; Nance, S.; O'Brien, J.; Wessells, V. M.; Goodspeed, A. E.; Talbot, J. C.; Amacher, S. L.; Jedlicka, P.; Black, J. C.; Costello, J. C.; Durbin, A. D.; Artinger, K. B.; Ford, H. L.

doi:10.1101/2021.04.25.439216

bioRxiv · 10.1101/2021.04.25.439216

SIX1 is a master regulator of the Rhabdomyosarcoma undifferentiated state

Hsu, J. Y.·Nance, S.·O'Brien, J.·Wessells, V. M.·Goodspeed, A. E.·Talbot, J. C.·Amacher, S. L.·Jedlicka, P.·Black, J. C.·Costello, J. C.·Durbin, A. D.·Artinger, K. B.·Ford, H. L.

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Abstract

Rhabdomyosarcoma (RMS) is a pediatric skeletal muscle sarcoma characterized by the expression of the myogenic-lineage transcription factors (TF) MYOD1 and MYOG. Despite high expression of these TFs, RMS cells fail to terminally differentiate, suggesting the presence of factors that alter their function. Here, we demonstrate that the developmental TF, SIX1, is highly expressed in RMS and is critical to maintain a muscle progenitor-like state. SIX1 loss induces terminal differentiation of RMS cells into myotube-like cells and dramatically impedes tumor growth in vivo. We show that SIX1 maintains the RMS undifferentiated state by controlling enhancer activity and MYOD1 occupancy at loci more permissive to tumor growth over terminal muscle differentiation. Finally, we demonstrate that a gene signature derived from SIX1 loss correlates with differentiation status in RMS and predicts RMS progression in human disease. Our findings demonstrate a master regulatory role for SIX1 in the repression of RMS differentiation via genome-wide alterations in MYOD1-mediated transcription. HighlightsO_LISIX1 prevents differentiation in RMS while it promotes differentiation during normal development C_LIO_LIFN-RMS are highly dependent on SIX1 for growth in both zebrafish and mouse xenograft models C_LIO_LILoss of SIX1 alters the transcriptional landscape of RMS cells, inducing a growth to differentiation switch C_LIO_LISIX1 knockdown in FN-RMS causes reduced super enhancer-based activity at stem-related genes and enhanced MYOD1 binding to differentiation loci, resulting in the activation of a myogenic differentiation program C_LIO_LIA gene signature derived from SIX1 loss strongly correlates with myogenic differentiation status and is predictive of advanced RMS. C_LI

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Hsu, J. Y., Nance, S., O'Brien, J., Wessells, V. M., Goodspeed, A. E., Talbot, J. C., Amacher, S. L., Jedlicka, P., Black, J. C., Costello, J. C., Durbin, A. D., Artinger, K. B., Ford, H. L.. 2021-04-26. SIX1 is a master regulator of the Rhabdomyosarcoma undifferentiated state. https://doi.org/10.1101/2021.04.25.439216

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