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bioRxiv · 10.1101/2021.04.21.440837

Point-localized and site-specific membrane potential optical recording by engineered single fluorescent nanodiscs

Grupi, A.·Shapira, Z.·Degani-Katzav, N.·Yudovich, S.·Weiss, S.
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Abstract

Nanodisc technology was implemented as a platform for voltage nanosensors. A FRET-based voltage sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine (DPA) was developed and utilized to optically record membrane potentials on the single nanodisc level. Ensemble- and single- nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA-A antibodies allowed for site specific membrane potential measurements from post synaptic sites.

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Grupi, A., Shapira, Z., Degani-Katzav, N., Yudovich, S., Weiss, S.. 2021-04-22. Point-localized and site-specific membrane potential optical recording by engineered single fluorescent nanodiscs. https://doi.org/10.1101/2021.04.21.440837

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