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bioRxiv · 10.1101/2021.04.19.440507

Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds

Clough, M.·Chen, I. A.·Park, S.-W.·Ahrens, A. M.·Stirman, J. N.·Smith, S. L.·Chen, J. L.
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Abstract

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

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Clough, M., Chen, I. A., Park, S.-W., Ahrens, A. M., Stirman, J. N., Smith, S. L., Chen, J. L.. 2021-04-20. Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds. https://doi.org/10.1101/2021.04.19.440507

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