Precision Genome and Base Editing by Regulating mRNA Nuclear Export Using Selective Inhibitors of Nuclear Export

Cui, Y.; Wang, S.; Ma, T.; Yu, P.; Chen, J.; Guo, T.; Meng, G.; Jiang, B.; Dong, J.; Liu, J.

doi:10.1101/2021.04.17.440169

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bioRxiv · 10.1101/2021.04.17.440169

Precision Genome and Base Editing by Regulating mRNA Nuclear Export Using Selective Inhibitors of Nuclear Export

Cui, Y.·Wang, S.·Ma, T.·Yu, P.·Chen, J.·Guo, T.·Meng, G.·Jiang, B.·Dong, J.·Liu, J.

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Abstract

CRISPR-based genome engineering tools are associated with off-target effects that constitutively active Cas9 protein may instigate. In the present study, we screened for irreversible small molecule off-switches of CRISPR-Cas9 and discovered that selective inhibitors of nuclear export (SINEs) could inhibit the cellular activity of CRISPR-Cas9 by interfering with the nuclear export of Cas9 mRNA. We subsequently found that SINEs, including an FDA-approved anticancer drug KPT330, could improve the specificities of CRISPR-Cas9-based genome and base editing tools in human cells.

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Cui, Y., Wang, S., Ma, T., Yu, P., Chen, J., Guo, T., Meng, G., Jiang, B., Dong, J., Liu, J.. 2021-04-17. Precision Genome and Base Editing by Regulating mRNA Nuclear Export Using Selective Inhibitors of Nuclear Export. https://doi.org/10.1101/2021.04.17.440169

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