Elevated anti-SARS-CoV-2 antibodies and IL-6, IL-8, MIP-1β, early predictors of severe COVID-19.

Codina, H.; Vieitez, I.; Gutierrez-Valencia, A.; Skouridou, V.; Martínez, C.; Patiño, L.; Botero-Gallego, M.; Trujillo-Rodríguez, M.; Serna-Gallego, A.; Muñoz-Muela, E.; M. Bobillo, M.; Pérez, A.; Cabrera-Alvar, J. J.; Crespo, M.; O'Sullivan, C. K.; Ruiz-Mateos, E.; Poveda, E.

doi:10.1101/2021.04.13.439586

bioRxiv · 10.1101/2021.04.13.439586

Elevated anti-SARS-CoV-2 antibodies and IL-6, IL-8, MIP-1β, early predictors of severe COVID-19.

Codina, H.·Vieitez, I.·Gutierrez-Valencia, A.·Skouridou, V.·Martínez, C.·Patiño, L.·Botero-Gallego, M.·Trujillo-Rodríguez, M.·Serna-Gallego, A.·Muñoz-Muela, E.·M. Bobillo, M.·Pérez, A.·Cabrera-Alvar, J. J.·Crespo, M.·O'Sullivan, C. K.·Ruiz-Mateos, E.·Poveda, E.

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Abstract

BackgroundViral and host immune kinetics during acute COVID-19 and after remission of acute symptoms need better characterization. MethodsSARS-CoV-2 RNA, anti-SARS-CoV-2 IgA, IgM, and IgG antibodies, and pro-inflammatory cytokines were measured in sequential samples among hospitalized COVID-19 patients during acute infection and 6 months following diagnosis. Results24 laboratory-confirmed COVID-19 patients with mild/moderate and severe COVID-19 were included. Most were males 83%, median age of 61 years. 21% were admitted to the ICU and 8 of them (33.3%) met criteria for severe COVID-19 disease. A delay in SARS-CoV-2 levels decline during the first 6 days of follow-up and viral load persistence until month 3 were related with severe COVID-19, but not viral load levels at the diagnosis. Higher levels of anti-SARS-CoV-2 IgA, IgM, IgG and the cytokines IL-6, IL-8 and MIP-1{beta} at the diagnosis time were related with severe COVID-19 outcome. Higher levels of MIP-1{beta}, IL-1{beta}, MIP-1 and IFN-{gamma} were observed at month 1/3 during mild/moderate disease compared to severe COVID-19. IgG persisted at low levels after 6 months of diagnosis. ConclusionsHigher concentrations of IgA, IgM, and IgG, and IL-6, IL-8 and MIP-1{beta} are identified as early predictors of COVID-19 severity, but not SARS-CoV-2 RNA levels at diagnosis.

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Codina, H., Vieitez, I., Gutierrez-Valencia, A., Skouridou, V., Martínez, C., Patiño, L., Botero-Gallego, M., Trujillo-Rodríguez, M., Serna-Gallego, A., Muñoz-Muela, E., M. Bobillo, M., Pérez, A., Cabrera-Alvar, J. J., Crespo, M., O'Sullivan, C. K., Ruiz-Mateos, E., Poveda, E.. 2021-04-13. Elevated anti-SARS-CoV-2 antibodies and IL-6, IL-8, MIP-1β, early predictors of severe COVID-19.. https://doi.org/10.1101/2021.04.13.439586

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