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bioRxiv · 10.1101/2021.04.12.439451

Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domain

Qiu, R.·Zhang, J.·Rotty, J. D.·Xiang, X.
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Abstract

Cytoplasmic dynein is activated by dynactin and cargo adapters in vitro, and the activation also needs LIS1 (Lissencephaly 1) in vivo. How this process is regulated remains unclear. Here we found in Aspergillus nidulans that a dynein AAA4 arginine-finger mutation bypasses the requirement of LIS1 for dynein activation driven by the early endosomal adapter HookA. As the AAA4 arginine-finger is implicated in AAA3 ATP hydrolysis, we examined AAA3 mutants defective in ATP binding and hydrolysis respectively. Astonishingly, blocking AAA3 ATP hydrolysis allows dynein activation by dynactin in the absence of LIS1 or HookA. As a consequence, dynein accumulates at microtubule minus ends while early endosomes stay near the plus ends. On the other hand, blocking AAA3 ATP binding abnormally prevents LIS1 from being dissociated from dynein upon motor activation. Thus, the AAA3 ATPase cycle regulates the coordination between dynein activation and cargo binding as well as the dynamic dynein-LIS1 interaction.

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Qiu, R., Zhang, J., Rotty, J. D., Xiang, X.. 2021-04-12. Dynein activation in vivo is regulated by the nucleotide states of its AAA3 domain. https://doi.org/10.1101/2021.04.12.439451

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