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bioRxiv · 10.1101/2021.04.12.439409

Crystal sturctures of Scone, pseudosymmetric folding of a symmetric designer protein

Mylemans, B.·Killian, T.·Vandebroek, L.·Van Meervelt, L.·Tame, J. R. H.·Parac-vogt, T. N.·Voet, A. R. D.
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Abstract

Recent years have seen a raise in the development of computational proteins including symmetric ones. We recently developed a nine-fold symmetric {beta}-propeller protein named Cake. Here we wanted to further engineer this protein to a three-fold symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography however revealed the structure of both designs to adopt an eight-fold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon addition of a polyoxometalate highlighting the usefulness of these molecules as a crystallisation additive.

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Mylemans, B., Killian, T., Vandebroek, L., Van Meervelt, L., Tame, J. R. H., Parac-vogt, T. N., Voet, A. R. D.. 2021-04-12. Crystal sturctures of Scone, pseudosymmetric folding of a symmetric designer protein. https://doi.org/10.1101/2021.04.12.439409

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