Recombinant binder of sperm protein1 (rec-BSP1) as a potent fertility factor mediates its effect on embryo production

Bag, S.; Ansari, S.; Alka Turuk, A.; Mahanandia, N. C.; Saini, S.; Biswal, S. K.; Singh, S. K.; Malik, P.; Kumar, S.; Malakar, D.

doi:10.1101/2021.04.12.437362

bioRxiv · 10.1101/2021.04.12.437362

Recombinant binder of sperm protein1 (rec-BSP1) as a potent fertility factor mediates its effect on embryo production

Bag, S.·Ansari, S.·Alka Turuk, A.·Mahanandia, N. C.·Saini, S.·Biswal, S. K.·Singh, S. K.·Malik, P.·Kumar, S.·Malakar, D.

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Abstract

ObjectiveTo understand the effect of recombinant BSP1 (rec-BSP1) on in vitro capacitation of sperm and fertilization study Method(s)Articles were screened for reports including rec-BSP1, Capacitation, in vitro fertilization InterventionNone Main Outcome Measure(s)Reproductive outcomes, effect on gametes and embryos Result(s)Here we report an optimization of condition for rec-BSP1 production which was used for in vitro capacitation and enhancement of buffalo embryo production. The sequence of the protein was used for multiple sequence alignment which has 99% similarity with PDC 109 protein. The expression of rec-BSP1 was carried out successfully with 1 mM IPTG at 160 C for 22 hrs and purified it in soluble form. The structure of rec-BSP1 was generated using 3D modelling and analysed its mode of binding with heparin and PC by molecular docking and the structural stability of rec-BSP1-PC and rec-BSP1-heparin complexes by using molecular dynamic (MD) simulation. The effect of rec-BSP1 was observed on in vitro capacitation of spermatozoa and buffalo blastocyst production. It was found that the rec-BSP1 enhanced the sperm motility at a concentration of 50 g/ml for 1 h of incubation without having any detrimental effect on the sperm morphology and a significant increase in blastocyst production at concentration of 50 g/ml rec-BSP1. Hence this finding represents a new insight and advance the prospective approach to develop a potential fertility factor in reproduction. Conclusion(s)The purified rec-BSP1 may enhance on male fertility and mediated its effect on in vitro blastocyst production in buffalo.

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Bag, S., Ansari, S., Alka Turuk, A., Mahanandia, N. C., Saini, S., Biswal, S. K., Singh, S. K., Malik, P., Kumar, S., Malakar, D.. 2021-04-13. Recombinant binder of sperm protein1 (rec-BSP1) as a potent fertility factor mediates its effect on embryo production. https://doi.org/10.1101/2021.04.12.437362

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