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bioRxiv · 10.1101/2021.04.08.439058

NaCT (SLC13A5) facilitates citrate import and metabolism under nutrient-limited conditions

Kumar, A.·Cordes, T.·Thalacker-Mercer, A. E.·Pajor, A. M.·Murphy, A. N.·Metallo, C. M.
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Abstract

Citrate lies at a critical node of metabolism linking tricarboxylic acid metabolism and fatty acid synthesis via acetyl-coenzyme A. Recent studies have linked the sodium citrate transporter (NaCT), encoded by SLC13A5, to dysregulated hepatic metabolism and pediatric epilepsy. To examine how NaCT-mediated citrate metabolism contributes to the pathophysiology of these diseases we applied 13C isotope tracing to SLC13A5-deficient hepatocellular carcinoma (HCC) cell lines and primary rat cortical neurons. Exogenous citrate contributed to intermediary metabolism at appreciable levels only under hypoxic conditions. In the absence of glutamine, citrate supplementation increased de novo lipogenesis and growth of HCC cells. Knockout of SLC13A5 in Huh7 cells compromised citrate uptake and catabolism. Citrate supplementation rescued Huh7 cell viability in response to glutamine deprivation and Zn2+ treatment, and these effects were mitigated by NaCT deficiency. Collectively, these findings demonstrate that NaCT-mediated citrate uptake is metabolically important under nutrient limited conditions and may facilitate resistance to metal toxicity.

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Kumar, A., Cordes, T., Thalacker-Mercer, A. E., Pajor, A. M., Murphy, A. N., Metallo, C. M.. 2021-04-10. NaCT (SLC13A5) facilitates citrate import and metabolism under nutrient-limited conditions. https://doi.org/10.1101/2021.04.08.439058

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