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bioRxiv · 10.1101/2021.04.08.438993

BIOMODULATORY EFFECT OF LOW INTENSITY LASER (830 nm.) IN NEURAL CELLS 9L/lacZ

Zabeu, A. M. C.·Carvalho, I. C.·Pacheco-Soares, C.·da Silva, N. S.
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Abstract

BackgroundCurrently, research is advancing low intensity laser (LIL) in central nervous system cells to available the benefits of this therapy in neurological disorders, and research seeks to establish the best LIL protocol in biological processes of neuronal tissue with the different energy wavelengths({lambda}), and exposure time (s) and frequency(Hz). The aim of this study is to check the biomodulatory effects of the LIL in neural cell culture. MethodsDiode laser {lambda} = 830 nm, power of 40 mW, continuous mode, applied in the cells lineage 9L/lacZ, with energy densities of 0.5, 1.5 and 3.0 J/cm2. Analysed 24 hours after irradiation, the results of the cell viability show the difference between the control and treated groups. ResultsIn the occurrence of apoptosis, no significant manifestation was observed between the control group compared with the irradiated one (P = 0.9956); there was a significant difference between apoptosis and death by necrosis has been between the control and treated groups (P <0.001). In the comet assay there was not difference. ConclusionsWith the aim of evaluating whether LIL promotes early activation of programmed cell death, of 9L/lacZ cells, in the proposed parameters of LIL, we observed that promoted an increase in the number of neural cells, highlighting the action of biomodulation; LIL did not promote the activation of apoptosis and did not any indication of DNA deterioration in the comet assay. The results of this study are indicative that the near infrared laser has a positive interaction with neuronal cells.

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Zabeu, A. M. C., Carvalho, I. C., Pacheco-Soares, C., da Silva, N. S.. 2021-04-09. BIOMODULATORY EFFECT OF LOW INTENSITY LASER (830 nm.) IN NEURAL CELLS 9L/lacZ. https://doi.org/10.1101/2021.04.08.438993

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