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bioRxiv · 10.1101/2021.04.01.438103

Interactome analysis of C. elegans synapses by TurboID-based proximity labeling

Artan, M.·Barratt, S.·Flynn, S. M.·Begum, F.·Skehel, M.·Nicolas, A.·de Bono, M.
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Abstract

Proximity labelling provides a powerful in vivo tool to characterize the proteome of sub-cellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labelling protocol for C. elegans. We use this protocol to characterise the proteomes of the worms gut, muscle, skin, and nervous system. We express TurboID exclusively in the pair of AFD neurons and show we can identify known and previously unknown proteins expressed selectively in AFD. We knock TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identify many known ELKS-1 interacting proteins as well as previously uncharacterised synaptic proteins. Versatile vectors, and the inherent advantage of C. elegans for biochemistry, make proximity labelling a valuable addition to the nematodes armory. TeaserWe optimize a TurboID proximity labeling protocol for C. elegans and use it to characterize tissue and synaptic proteomes

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Artan, M., Barratt, S., Flynn, S. M., Begum, F., Skehel, M., Nicolas, A., de Bono, M.. 2021-04-01. Interactome analysis of C. elegans synapses by TurboID-based proximity labeling. https://doi.org/10.1101/2021.04.01.438103

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