Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Viswanatha, R.; Mameli, E.; Rodiger, J.; Merckaert, P.; Feitosa-Suntheimer, F.; Colpitts, T. M.; Mohr, S. E.; Hu, Y.; Perrimon, N.

doi:10.1101/2021.03.29.437496

bioRxiv · 10.1101/2021.03.29.437496

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Viswanatha, R.·Mameli, E.·Rodiger, J.·Merckaert, P.·Feitosa-Suntheimer, F.·Colpitts, T. M.·Mohr, S. E.·Hu, Y.·Perrimon, N.

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Abstract

Mosquito-borne diseases present a worldwide public health burden. Genome-scale screening tools that could inform our understanding of mosquitos and their control are lacking. Here, we adapt a recombination-mediated cassette exchange system for delivery of CRISPR sgRNA libraries into cell lines from several mosquito species and perform pooled CRISPR screens in an Anopheles cell line. To implement this method, we engineered modified mosquito cell lines, validated promoters and developed bioinformatics tools for multiple mosquito species.

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Viswanatha, R., Mameli, E., Rodiger, J., Merckaert, P., Feitosa-Suntheimer, F., Colpitts, T. M., Mohr, S. E., Hu, Y., Perrimon, N.. 2021-03-29. Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos. https://doi.org/10.1101/2021.03.29.437496

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