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bioRxiv · 10.1101/2021.03.19.436090

Limited effects of m6A modification on mRNA partitioning into stress granules

Khong, A.·Matheny, T.·Huynh, T. N.·Babl, V.·Parker, R.
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Abstract

Recent studies have argued that the m6A modification of mRNAs promotes mRNA recruitment to stress granules through the interaction with YTHDF proteins (Anders et al., 2018; Ries et al., 2019). However, mRNAs that contain multiple m6A modified sites partition similarly into stress granules in both wild-type and m6A-deficient cells by single-molecule FISH suggesting m6A modifications play a minor role in mRNA partitioning into stress granules. Moreover, multiple linear regression analysis suggests m6A modification plays a minimal role in stress granule recruitment. Finally, the artificial tethering of 25 YTHDF proteins on reporter mRNAs leads to only a modest increase in mRNA partitioning to stress granules. These results indicate m6A modification makes a small, but measurable, contribution to recruiting specific mRNAs to stress granules.

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Khong, A., Matheny, T., Huynh, T. N., Babl, V., Parker, R.. 2021-03-19. Limited effects of m6A modification on mRNA partitioning into stress granules. https://doi.org/10.1101/2021.03.19.436090

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