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bioRxiv · 10.1101/2021.03.14.435354

Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase

Wang, W.·Dasetty, S.·Sarupria, S.·Blenner, M.
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Abstract

While thermophilic enzymes have thermostability desired for broad industrial applications, they can lose activity at ambient temperatures far from their optimal. Engineering cold activity into thermophilic enzymes has the potential to broaden the range of temperatures resulting in significant activity (i.e., decreasing the temperature dependence of kcat). Even though it has been widely suggested that cold temperature enzyme activity results from active flexibility that is at odds with the rigidity necessary for thermostable enzymes; however, directed evolution experiments have shown us these properties are not mutually exclusive. In this study, rational protein engineering was used to introduce flexibility inducing mutations around the active sites of Geobacillus thermocatenulatus lipase (GTL). Two mutants were found to have enhanced specific activity compared to wild-type at temperatures between 283 K to 363 K with p-nitrophenol butyrate but not with larger substrates. Kinetics assay revealed both mutations resulted in psychrophilic traits, such as lower activation enthalpy and more negative entropy values compared to wild type in all substrates. Furthermore, the mutants had significantly improved thermostability compared to wild type enzyme, which proves that it is feasible to improve the cold activity without trade-off. Our study provides insight into the enzyme cold adaptation mechanism and design principles for engineering cold activity into thermostable enzymes.

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Wang, W., Dasetty, S., Sarupria, S., Blenner, M.. 2021-03-15. Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase. https://doi.org/10.1101/2021.03.14.435354

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