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bioRxiv · 10.1101/2021.03.13.434679

Age-related increase in matrix stiffness downregulates α-Klotho in chondrocytes and induces cartilage degeneration

Abstract

Enhanced mechanistic insight into age-related knee osteoarthritis (KOA) is an essential step to promote successful translation of animal research to bedside interventions. To this end, the goal of these studies was to interrogate molecular mechanisms driving age-related KOA in a mouse model and correspond findings to human knee cartilage. Unbiased mass spectrometry proteomics of cartilage tissue revealed PI3K/Akt signaling was the predominant pathway disrupted over time in male, but not female, mice. This finding was consistent with a significantly accelerated KOA progression in males when compared to female counterparts. In probing for upstream regulators of these age-dependent alterations, we found that -Klotho, a suppressor of PI3K/Akt signaling and potent longevity protein, significantly decreased with aging in both mouse and human knee cartilage. Upstream of these alterations, we found that age-related increases in matrix stiffness initiated a cascade of altered nuclear morphology and downregulated -Klotho expression, ultimately impairing chondrocyte health. Conversely, reducing matrix stiffness increased -Klotho expression in chondrocytes, thus enhancing their chondrogencity and cartilage integrity. Collectively, our findings establish a novel mechanistic link between age-related alterations in ECM biophysical properties and regulation of cartilage health by -Klotho.

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BibTeXRIS

Iijima, H., Gilmer, G., Wang, K., Bean, A., He, Y., Lin, H., Evans, C., Ambrosio, F.. 2021-03-14. Age-related increase in matrix stiffness downregulates α-Klotho in chondrocytes and induces cartilage degeneration. https://doi.org/10.1101/2021.03.13.434679

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