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bioRxiv · 10.1101/2021.03.11.435015

Two distinct mechanisms underlie dosage sensitivity in Pumilio1-associated diseases

Abstract

Mutations in the RNA-binding protein (RBP) Pumilio1 (PUM1) can cause dramatically different phenotypes. We previously noted that phenotypic severity tracked with protein dosage: a mild mutation that reduces PUM1 levels by 25% causes late-onset ataxia, whereas PUM1 haploinsufficiency causes developmental delay and seizures. Why this difference in expression should cause such different phenotypes has been unclear: PUM1 targets are de-repressed to equal degrees in both cases, and the more severe mutation does not hinder PUM1s RNA-binding ability. We therefore developed a PUM1 interactome in the murine brain. We find that mild PUM1 loss de-represses PUM1-specific targets, but PUM1 haploinsufficiency disrupts several interactors and regulation of their targets. We validated these phenomena in patient-derived cell lines and show that normalizing PUM1 levels restores interactors and their targets to proper levels. We therefore propose that dosage sensitivity does not necessarily reflect a linear change in protein abundance but can involve distinct mechanisms. Studying the interactors of RBPs in vivo will be necessary to understand their functions in neurological diseases.

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BibTeXRIS

Botta, S., de Prisco, N., Chemiakine, A., Cabaj, M., Brandt, V. L., Patel, P., Soni, R. K., Gennarino, V. A.. 2021-03-12. Two distinct mechanisms underlie dosage sensitivity in Pumilio1-associated diseases. https://doi.org/10.1101/2021.03.11.435015

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