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bioRxiv · 10.1101/2021.03.11.434985

Spatial Epigenome Sequencing at Tissue Scale and Cellular Level

Deng, Y.·Zhang, D.·Liu, Y.·Su, G.·Enninful, A.·Bai, Z.·Fan, R.
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Abstract

Spatial biology is emerging as a new frontier of biomedical research in development and disease, but currently limited to transcriptome and a panel of proteins. Here we present spatial epigenome profiling for three histone modifications (H3K27me3, H3K4me3, H3K27ac) via next-generation sequencing by combining in-tissue CUT&Tag chemistry and microfluidic deterministic barcoding. Spatial chromatin states in mouse embryos or olfactory bulbs revealed tissue type-specific epigenetic regulations, in concordance with ENCODE reference data, but providing spatially resolved genome-wide profiles at tissue scale. Using fluorescence imaging to identify the tissue pixels (20m) each containing one nucleus allowed us to extract single-cell epigenomes in situ. Spatial chromatin state profiling in tissue may enable unprecedented opportunities to study epigenetic regulation, cell function and fate decision in normal physiology and pathogenesis.

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Deng, Y., Zhang, D., Liu, Y., Su, G., Enninful, A., Bai, Z., Fan, R.. 2021-03-12. Spatial Epigenome Sequencing at Tissue Scale and Cellular Level. https://doi.org/10.1101/2021.03.11.434985

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