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bioRxiv · 10.1101/2021.03.09.434205

RS-FISH: Precise, interactive and scalable smFISH spot detection using Radial Symmetry

Bahry, E.·Breimann, L.·Epstein, L.·Kolyvanov, K.·Harrington, K.·Lionnet, T.·Preibisch, S.
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Abstract

Fluorescent in-situ hybridization (FISH)-based methods are powerful tools to study molecular processes with subcellular resolution, relying on accurate identification and localization of diffraction-limited spots in microscopy images. We developed the Radial Symmetry-FISH (RS-FISH) software that accurately, robustly, and quickly detects single-molecule spots in two and three dimensions, making it applicable to several key assays, including single-molecule FISH (smFISH), spatial transcriptomics, and spatial genomics. RS-FISH allows interactive parameter tuning and scales to large sets of images as well as tera-byte sized image volumes such as entire brain scans using straight-forward distributed processing on workstations, clusters, and in the cloud.

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Bahry, E., Breimann, L., Epstein, L., Kolyvanov, K., Harrington, K., Lionnet, T., Preibisch, S.. 2021-03-09. RS-FISH: Precise, interactive and scalable smFISH spot detection using Radial Symmetry. https://doi.org/10.1101/2021.03.09.434205

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