The dynamics of cell-free DNA from urine and blood after a full marathon

Shishikura, Y.; Tokinoya, K.; Aita, Y.; Sekine, N.; Sugasawa, T.; Yoshida, Y.; Kosaki, K.; Kumamoto, S.; Ishikura, K.; Kuji, T.; Kawakami, Y.; Nabekura, Y.; Maeda, S.; Takekoshi, K.

doi:10.1101/2021.03.06.434188

bioRxiv · 10.1101/2021.03.06.434188

The dynamics of cell-free DNA from urine and blood after a full marathon

Shishikura, Y.·Tokinoya, K.·Aita, Y.·Sekine, N.·Sugasawa, T.·Yoshida, Y.·Kosaki, K.·Kumamoto, S.·Ishikura, K.·Kuji, T.·Kawakami, Y.·Nabekura, Y.·Maeda, S.·Takekoshi, K.

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Abstract

PurposeCell-free DNA (cfDNA) has been investigated as a minimally invasive biomarker for many diseases, particularly cancer. An increase in cfDNA has been observed during exercise. Neutrophil extracellular traps (NETs) may be the origin of cfDNA in response to acute exercise, but the mechanisms of generation of cfDNA during exercise remain unclear. In this study we investigated the dynamics of serum and urinary cfDNA levels and determined the relevance of other biomarkers to serum and urinary cfDNA levels and fragment size after a full marathon. MethodsSamples were collected from 23 healthy male subjects. Blood and urine samples were collected before and immediately, two hours, and one day after the full marathon. The measurements included serum and urinary cfDNA, creatine kinase, myoglobin, creatinine, white blood cells, platelets, and lactoferrin from blood, and amylase, albumin, and creatinine from urine. ResultsSerum and urinary cfDNA levels increased after a full marathon. Creatine kinase, myoglobin, and creatinine in blood, and albumin and creatinine in urine also increased significantly after a full marathon. Serum cfDNA showed peak values about 180 bp after the full marathon. Values over 1000 bp were present at two hours post-marathon. Urinary cfDNA showed peak values from 35 bp to 50 bp after the full marathon. Values over 1000 bp appeared at Immediately and two hours post marathon. ConclusionThis study revealed that both serum and urinary cfDNA levels transiently increased after a full marathon. In addition, these cfDNA fragment varied in size.

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Shishikura, Y., Tokinoya, K., Aita, Y., Sekine, N., Sugasawa, T., Yoshida, Y., Kosaki, K., Kumamoto, S., Ishikura, K., Kuji, T., Kawakami, Y., Nabekura, Y., Maeda, S., Takekoshi, K.. 2021-03-08. The dynamics of cell-free DNA from urine and blood after a full marathon. https://doi.org/10.1101/2021.03.06.434188

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