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bioRxiv · 10.1101/2021.02.26.433019

A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

Sanchez, M.·Palomba, E.·Tirelli, V.·de Alteriis, E.·Landi, C.·Mazzoleni, S.
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Abstract

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

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Sanchez, M., Palomba, E., Tirelli, V., de Alteriis, E., Landi, C., Mazzoleni, S.. 2021-02-26. A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor. https://doi.org/10.1101/2021.02.26.433019

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