Self-contained and modular structured illumination microscope

Van den Eynde, R.; Vandenberg, W.; Hugelier, S.; Bouwens, A.; Hofkens, J.; Müller, M.; Dedecker, P.

doi:10.1101/2021.02.25.432746

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bioRxiv · 10.1101/2021.02.25.432746

Self-contained and modular structured illumination microscope

Van den Eynde, R.·Vandenberg, W.·Hugelier, S.·Bouwens, A.·Hofkens, J.·Müller, M.·Dedecker, P.

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Abstract

We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our instrument, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments.

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Van den Eynde, R., Vandenberg, W., Hugelier, S., Bouwens, A., Hofkens, J., Müller, M., Dedecker, P.. 2021-02-25. Self-contained and modular structured illumination microscope. https://doi.org/10.1101/2021.02.25.432746

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