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bioRxiv · 10.1101/2021.02.24.432807

Modeling SARS-CoV-2 nsp1-5'-UTR complex via the extended ensemble simulations

Sakuraba, S.·Qilin, X.·Kasahara, K.·Iwakiri, J.·Kono, H.
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Abstract

Nonstructural protein 1 (nsp1) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a 180-residue protein that blocks translation of host mRNAs in SARS-CoV-2-infected cells. Although it is known that SARS-CoV-2s own RNA evades nsp1s host translation shutoff, the molecular mechanism underlying the evasion was poorly understood. We performed an extended ensemble molecular dynamics simulation to investigate the mechanism of the viral RNA evasion. Simulation results showed that the stem loop structure of the SARS-CoV-2 RNA 5-untranslated region (SL1) is recognized by both nsp1s globular region and intrinsically disordered region. The recognition presumably enables selective translation of viral RNAs. Cluster analysis of the binding mode and detailed analysis of the binding poses revealed several residues involved in the SL1 recognition mechanism. The simulation results imply that the nsp1 C-terminal helices are lifted from the 40S ribosome upon the binding of SL1 to nsp1, unblocking translation of the viral RNA.

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Sakuraba, S., Qilin, X., Kasahara, K., Iwakiri, J., Kono, H.. 2021-02-25. Modeling SARS-CoV-2 nsp1-5'-UTR complex via the extended ensemble simulations. https://doi.org/10.1101/2021.02.24.432807

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