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bioRxiv · 10.1101/2021.02.24.432491

Longitudinal high-resolution imaging through a flexible intravital imaging window

Jacquemin, G.·Benavente-Diaz, M.·Djaber, S.·Bore, A.·Dangles-Marie, V.·Surdez, D.·Tajbakhsh, S.·Fre, S.·Lloyd-Lewis, B.
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Abstract

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues. One-sentence summaryThis study presents a versatile, fully flexible imaging window that acts as an implantable transparent second skin for small laboratory animal in vivo imaging.

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Jacquemin, G., Benavente-Diaz, M., Djaber, S., Bore, A., Dangles-Marie, V., Surdez, D., Tajbakhsh, S., Fre, S., Lloyd-Lewis, B.. 2021-02-24. Longitudinal high-resolution imaging through a flexible intravital imaging window. https://doi.org/10.1101/2021.02.24.432491

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