Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species

Tomaszewska, P.; Vorontsova, M. S.; Renvoize, S. A.; Ficinski, S. Z.; Tohme, J.; Schwarzacher, T.; Castiblanco, V.; de Vega, J. J.; Mitchell, R. A. C.; Heslop-Harrison, P.

doi:10.1101/2021.02.19.431966

bioRxiv · 10.1101/2021.02.19.431966

Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species

Tomaszewska, P.·Vorontsova, M. S.·Renvoize, S. A.·Ficinski, S. Z.·Tohme, J.·Schwarzacher, T.·Castiblanco, V.·de Vega, J. J.·Mitchell, R. A. C.·Heslop-Harrison, P.

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Abstract

Background and AimsDiploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa and now planted worldwide are important for food security and the environment, often being planted in marginal lands. We aimed to characterize the nature of their genomes, the repetitive DNA, and the genome composition of polyploids, leading to a model of the evolutionary pathways within the group including many apomictic species. MethodsSome 362 forage grass accessions from international germplasm collections were studied, and ploidy determined using an optimized flow cytometry method. Whole-genome survey sequencing and molecular cytogenetic analysis with in situ hybridization to chromosomes were used to identify chromosomes and genomes in Urochloa accessions belonging to the different agamic complexes. Key ResultsGenome structures are complex and variable, with multiple ploidies and genome compositions within the species, and no clear geographical patterns. Sequence analysis of nine diploid and polyploid accessions enabled identification of abundant genome-specific repetitive DNA motifs. In situ hybridization with a combination of repetitive DNA and genomic DNA probes, identified evolutionary divergence and allowed us to discriminate the different genomes present in polyploids. ConclusionsWe suggest a new coherent nomenclature for the genomes present. We develop a model of evolution at the whole-genome level in diploid and polyploid accessions showing processes of grass evolution. We support the retention of narrow species concepts for U. brizantha, U. decumbens, and U. ruziziensis. The results and model will be valuable in making rational choices of parents for new hybrids, assist in use of the germplasm for breeding and selection of Urochloa with improved sustainability and agronomic potential, and will assist in measuring and conserving biodiversity in grasslands.

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Tomaszewska, P., Vorontsova, M. S., Renvoize, S. A., Ficinski, S. Z., Tohme, J., Schwarzacher, T., Castiblanco, V., de Vega, J. J., Mitchell, R. A. C., Heslop-Harrison, P.. 2021-02-19. Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species. https://doi.org/10.1101/2021.02.19.431966

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