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bioRxiv · 10.1101/2021.02.15.431295

Accuracy of the pneumatic method for estimating xylem vulnerability to embolism in temperate diffuse-porous tree species

Paligi, S. S.·Link, R. M.·Isaza, E.·Bittencourt, P.·Sarmento Cabral, J.·Jansen, S.·Oliveira, R. S.·Pereira, L.·Schuldt, B.
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Abstract

O_LIThe increasing frequency of global change-type droughts has created a need for fast, accurate and widely applicable techniques for estimating xylem embolism resistance to improve forecasts of future forest changes. C_LIO_LIWe used data from 12 diffuse-porous temperate tree species covering a wide range of xylem safety to compare the pneumatic and flow-centrifuge method for constructing xylem vulnerability curves. We evaluated the agreement between parameters estimated with both methods and the sensitivity of pneumatic measurements to the measurement duration. C_LIO_LIThe agreement between xylem water potentials at 50% air discharged (PAD) estimated with the Pneumatron and 50% loss of hydraulic conductivity (PLC) estimated with the flow-centrifuge method was high (mean signed deviation: 0.12 MPa, Pearson correlation: 0.96 after 15 sec of gas extraction). However, the relation between the estimated slopes was more variable, resulting in lower agreement in xylem water potential at 12% and 88% PAD/PLC. All parameters were sensitive to the duration of the pneumatic measurement, with highest overall agreement between methods after 16 sec. C_LIO_LIWe conclude that, if applied correctly, the pneumatic method enables fast and inexpensive estimations of embolism resistance for a wide range of temperate, diffuse-porous species, which makes it attractive for predicting plant performance under climate change. C_LI

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Paligi, S. S., Link, R. M., Isaza, E., Bittencourt, P., Sarmento Cabral, J., Jansen, S., Oliveira, R. S., Pereira, L., Schuldt, B.. 2021-02-16. Accuracy of the pneumatic method for estimating xylem vulnerability to embolism in temperate diffuse-porous tree species. https://doi.org/10.1101/2021.02.15.431295

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