High-Intensity Interval Training Remodels the Proteome and Acetylome of Human Skeletal Muscle

Hostrup, M.; Lemminger, A. K.; Stocks, B.; Gonzalez-Franquesa, A.; Larsen, J. K.; Thomassen, M.; Weinert, B.; Bangsbo, J.; Deshmukh, A. S.

doi:10.1101/2021.02.10.430602

bioRxiv · 10.1101/2021.02.10.430602

High-Intensity Interval Training Remodels the Proteome and Acetylome of Human Skeletal Muscle

Hostrup, M.·Lemminger, A. K.·Stocks, B.·Gonzalez-Franquesa, A.·Larsen, J. K.·Thomassen, M.·Weinert, B.·Bangsbo, J.·Deshmukh, A. S.

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Abstract

Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health. Impact statementHostrup and colleagues identify global proteomic and acetylomic adaptations to high-intensity interval training, demonstrating adaptations to processes regulating metabolism and contraction.

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Hostrup, M., Lemminger, A. K., Stocks, B., Gonzalez-Franquesa, A., Larsen, J. K., Thomassen, M., Weinert, B., Bangsbo, J., Deshmukh, A. S.. 2021-02-10. High-Intensity Interval Training Remodels the Proteome and Acetylome of Human Skeletal Muscle. https://doi.org/10.1101/2021.02.10.430602

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