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bioRxiv · 10.1101/2021.02.08.430300

Pumilio protects Xbp1 mRNA from regulated Ire1-dependent decay

Cairrao, F.·Santos, C.·Le-Thomas, A.·Marsters, S.·Ashkenazi, A.·Domingos, P.
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Abstract

The unfolded protein response (UPR) maintains homeostasis of the endoplasmic reticulum(ER). Residing in the ER membrane, the UPR mediator Ire1 deploys its cytoplasmic kinase-endoribonuclease domain to activate the key UPR transcription factor Xbp1 through non-conventional splicing of Xbp1 mRNA. Ire1 also degrades diverse ER-targeted mRNAs through regulated Ire1-dependent decay (RIDD), but how it spares Xbp1 mRNA from this decay is unknown. We identified binding sites for the RNA-binding protein Pumilio in the 3UTR Drosophila Xbp1. In the developing Drosophila eye, Pumilio bound both the Xbp1unspliced and Xbp1spliced mRNAs, but only Xbp1spliced was stabilized by Pumilio. Furthermore, Pumilio displayed Ire1 kinase-dependent phosphorylation during ER stress, which was required for its stabilization of Xbp1spliced. Human IRE1 could directly phosphorylate Pumilio, and phosphorylated Pumilio protected Xbp1spliced mRNA against RIDD. Thus, Ire1-mediated phosphorylation enables Pumilio to shield Xbp1spliced from RIDD. These results uncover an important and unexpected regulatory link between an RNA-binding protein and the UPR.

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Cairrao, F., Santos, C., Le-Thomas, A., Marsters, S., Ashkenazi, A., Domingos, P.. 2021-02-08. Pumilio protects Xbp1 mRNA from regulated Ire1-dependent decay. https://doi.org/10.1101/2021.02.08.430300

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