Increasing age is independently associated with higher free water in non-active MS brain - A multi-compartment analysis using FAST-T2

Zhou, L.; Li, Y.; Wang, X. H.; Sweeney, E.; Zhang, H.; Tanzi, E. B.; Prince, J.; Su-Ortiz, V. A.; Gauthier, S. A.; Nguyen, T. D.

doi:10.1101/2021.02.01.429067

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bioRxiv · 10.1101/2021.02.01.429067

Increasing age is independently associated with higher free water in non-active MS brain - A multi-compartment analysis using FAST-T2

Zhou, L.·Li, Y.·Wang, X. H.·Sweeney, E.·Zhang, H.·Tanzi, E. B.·Prince, J.·Su-Ortiz, V. A.·Gauthier, S. A.·Nguyen, T. D.

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Abstract

PurposeTo explore the relationship between the cerebral cortical perivascular space (PVS) and aging in non-active MS subjects by using the multi-echo T2 relaxometry based cerebrospinal fluid fraction (CSFF) map. MethodsMulti-echo spiral T2 data from 111 subjects with non-active multiple sclerosis (MS) were retrospectively investigated by fitting the T2 data into a three-compartment model, the three water compartments including myelin water, intra-extracellular water, and cerebrospinal fluid. Segmentation of T1w image was performed to get the region of interest (ROI) in cerebral cortical regions. The white matter lesion segmentation was conducted using a convolutional neural network (CNN) based segmentation tool. The CSFF in the ROIs were correlated with age by controlling the gender, white matter hyperintensity lesion burden, and MS disease duration. Multiple linear models were created for the analysis of aging effect on the CSFF. ResultsThe ROI analysis shows that the CSFF in the cerebral cortical regions (temporal, occipital, parietal, front, hippo, and mtl) are significantly linear increasing with age (p<0.01). The intra-extracellular water fraction (IEWF) in the ROIs are significantly linear decreasing (p<0.01). ConclusionThe multi-echo T2 based three-compartment model can be used to quantify the CSFF. The linear increase of CSF water contents in the cerebral cortical regions indicates increased perivascular space load in cortex with aging. The quantification of CSFF may provide a way to understand the glymphatic clearance function in aging and neurodegenerations. HighlightsO_LIMR T2 relaxometry is a valid method to quantify the cerebrospinal fluid fraction (CSFF) in cerebral cortical regions C_LIO_LIThe CSFF in the cerebral cortical regions are positively correlated with age by controlling the white matter lesion load in non-active MS subjects. C_LIO_LIQuantification of cerebral CSFF may reflect the perivascular space load in cortex and better interpret the disease progression in neurodegenerative disease, such as MS. C_LI

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Zhou, L., Li, Y., Wang, X. H., Sweeney, E., Zhang, H., Tanzi, E. B., Prince, J., Su-Ortiz, V. A., Gauthier, S. A., Nguyen, T. D.. 2021-02-02. Increasing age is independently associated with higher free water in non-active MS brain - A multi-compartment analysis using FAST-T2. https://doi.org/10.1101/2021.02.01.429067

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