Neuronal morphology and physiology, functional brain networks and memory in temporal lobe epilepsy

Douw, L.; Nissen, I. A.; Fitzsimmons, S. M.; Hillebrand, A.; van Straaten, E. C.; Stam, C. J.; de Witt Hamer, P. C.; Baayen, J. C.; Klein, M.; Reijneveld, J. C.; Heyer, D. B.; Verhoog, M. B.; Wilbers, R.; Hunt, S.; Mansvelder, H. D.; Geurts, J. J.; de Kock, C. P.; Goriounova, N. A.

doi:10.1101/2021.01.31.428369

bioRxiv · 10.1101/2021.01.31.428369

Neuronal morphology and physiology, functional brain networks and memory in temporal lobe epilepsy

Douw, L.·Nissen, I. A.·Fitzsimmons, S. M.·Hillebrand, A.·van Straaten, E. C.·Stam, C. J.·de Witt Hamer, P. C.·Baayen, J. C.·Klein, M.·Reijneveld, J. C.·Heyer, D. B.·Verhoog, M. B.·Wilbers, R.·Hunt, S.·Mansvelder, H. D.·Geurts, J. J.·de Kock, C. P.·Goriounova, N. A.

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Abstract

Temporal lobe epilepsy (TLE) patients are at risk of memory deficits, which have been linked to functional network disturbances, particularly of integration of the default mode network (DMN). However, the cellular substrates of functional network integration are unknown. We leverage a unique cross-scale dataset of therapy-resistant TLE patients, who underwent fMRI, MEG and/or neuropsychological testing before neurosurgery. fMRI and MEG underwent atlas-based connectivity analyses. Functional network centrality of the lateral middle temporal gyrus, part of the DMN, was used as a measure of local network integration. Subsequently, non-pathological cortical tissue from this region was used for single cell morphological and electrophysiological patch-clamp analysis, assessing integration in terms of total dendritic length and action potential rise speed. As could be hypothesized, greater network centrality related to better memory performance. Moreover, greater network centrality correlated with more integrative properties at the cellular level across patients. We conclude that individual differences in cognitively relevant functional network integration of a DMN region are mirrored by differences in cellular integrative properties of this region in TLE patients. These findings connect previously separate scales of investigation, increasing translational insight into focal pathology and large-scale network disturbances in TLE.

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Douw, L., Nissen, I. A., Fitzsimmons, S. M., Hillebrand, A., van Straaten, E. C., Stam, C. J., de Witt Hamer, P. C., Baayen, J. C., Klein, M., Reijneveld, J. C., Heyer, D. B., Verhoog, M. B., Wilbers, R., Hunt, S., Mansvelder, H. D., Geurts, J. J., de Kock, C. P., Goriounova, N. A.. 2021-02-01. Neuronal morphology and physiology, functional brain networks and memory in temporal lobe epilepsy. https://doi.org/10.1101/2021.01.31.428369

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