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bioRxiv · 10.1101/2021.01.21.427535

Nuclear stabilisation of p53 requires a functional nucleolar surveillance pathway

Abstract

The nucleolar surveillance pathway (NSP) monitors nucleolar fidelity and responds to nucleolar stresses (i.e., inactivation of ribosome biogenesis) by mediating the inhibitory binding of ribosomal proteins (RPs) to mouse double minute 2 homolog (MDM2), a nuclear-localised E3 ubiquitin ligase, which results in p53 accumulation. Inappropriate activation of the NSP has been implicated in the pathogenesis of collection of human diseases termed "ribosomopathies", while drugs that selectively activate the NSP are now in trials for cancer. Despite the clinical significance, the precise molecular mechanism(s) regulating the NSP remain poorly understood. Using genome-wide loss of function screens, we demonstrate the ribosome biogenesis (RiBi) axis as the most potent class of genes whose disruption stabilises p53. Furthermore, we identified a novel suite of genes critical for the NSP, including a novel mammalian protein implicated in 5S ribonucleoprotein particle (5S-RNP) biogenesis, HEATR3. By selectively disabling the NSP, we unexpectedly demonstrate that a functional NSP is required for the ability of all nuclear acting stresses tested, including DNA damage, to robustly induce p53 accumulation. Together, our data demonstrates that the NSP has evolved as the dominant central integrator of stresses that regulate nuclear p53 abundance, thus ensuring RiBi is hardwired to cellular proliferative capacity.

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BibTeXRIS

Hannan, K. M., Soo, P., Wong, M. S., Lee, J. K., Hein, N., Evers, M., Wysoke, K. D., Williams, T. D., Montellese, C., Smith, L., Al-Obaidi, S. J., Nunez-Villacis, L., Poh, P., Pavy, M., He, J.-S., Parsons, K. M., Diesch, J., Burgio, G., Ferreira, R., Feng, Z.-P., Gould, C. M., Madhamshettiwar, P. B., Flygare, J., Gonda, T. J., Simpson, K. J., Kutay, U., Pearson, R. B., Engel, C., Watkins, N., Hannan, R. D., George, A. J.. 2021-01-21. Nuclear stabilisation of p53 requires a functional nucleolar surveillance pathway. https://doi.org/10.1101/2021.01.21.427535

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